Search results for " ATP"
showing 10 items of 180 documents
Teritoriāla mārketinga un tēla veidošana Latvija.
2015
Krīstīnes Golubevas bakalaura darba tēma ir „Teritoriālā mārketinga un tēla veidošana Latvijā”. Darba mērķis ir, pamatojoties uz teritoriālā mārketinga un tēla veidošanas teoriju, Latvijas tēla atpazīstamības izpēti un analīzi, piedāvāt priekšlikumus Latvijas teritoriālā mārketinga un valsts tēla izpētes pilnveidošanai. Bakalaura darbs sastāv no ievada, trim nodaļām, secinājumiem un priekšlikumiem, izmantotās literatūras, avotu saraksta un 2 pielikumiem. Pirmajā daļā analizēti teritoriālā mārketinga teorētiskie aspekti: teritoriālā mārketinga definīcija un tās elementi, subjektu un mērķauditorijas loma teritoriālajā mārketingā un vides un konkurētspējas faktoru ietekme uz valsts mārketingu.…
Glucose-induced alterations of cytosolic free calcium in cultured rat tail artery vascular smooth muscle cells
1995
We have previously suggested that hyperglycemia per se may contribute to diabetic hypertensive and vascular disease by altering cellular ion content. To more directly investigate the potential role of glucose in this process, we measured cytosolic free calcium in primary cultures of vascular smooth muscle cells isolated from Sprague-Dawley rat tail artery before and after incubation with 5 (basal), 10, 15, and 20 mM glucose. Glucose significantly elevated cytosolic free calcium in a dose- and time-dependent manner, from 110.0 +/- 5.4 to 124.5 +/- 9.0, 192.7 +/- 20.4, and 228.4 +/- 21.9 nM at 5, 10, 15, and 20 mM glucose concentrations, respectively. This glucose-induced cytosolic free calci…
Insulin resistance and endogenous digoxin-like factor in obese hypertensive patients with glucose intolerance
1992
Hypertensive obese subjects with glucose intolerance have hyperinsulinaemia, insulin resistance and intracellular cation imbalance resulting in increased sodium content. The aim of our study was to assess in these patients plasma levels of endogenous digoxin-like factor (EDLF), an inhibitor of the sodium-pump mechanism. We studied 14 hypertensive and 12 normotensive subjects with obesity and glucose intolerance for fasting blood glucose, and plasma insulin, C-peptide and EDLF levels: the two groups were matched for age and BMI and were studied after a 2-week wash-out period from hypotensive drugs. Compared with normotensives, hypertensive subjects had higher plasma insulin levels, a greater…
A proton-translocating H+-ATPase is involved in C6 glial pH regulation.
1998
AbstractGlial cells extrude acid equivalents to maintain pHi. Although four mechanisms have been described so far, pHi-control under physiological conditions is still not sufficiently explained. We therefore investigated whether a H+-translocating ATPase is involved in glial pHi homeostasis using an established glial cell line (C6 glioma). In the absence of bicarbonate, the inhibition of H+-ATPases by NEM led to a pHi decrease. The application of a more specific inhibitor (NBD-Cl) showed that the H+-ATPase involved is of the vacuolar type. Inhibition went along with delayed cell swelling. Together with the fact that glial acidification was far more pronounced in Na+-free media, this may ser…
Inhibition by cellular vacuolar ATPase impairs human papillomavirus uncoating and infection.
2014
ABSTRACT Several viruses, including human papillomaviruses, depend on endosomal acidification for successful infection. Hence, the multisubunit enzyme vacuolar ATPase (V-ATPase), which is mainly responsible for endosome acidification in the cell, represents an attractive target for antiviral strategies. In the present study, we show that V-ATPase is required for human papillomavirus (HPV) infection and that uncoating/disassembly but not endocytosis is affected by V-ATPase inhibition. The infection inhibitory potencies of saliphenylhalamide, a proven V-ATPase inhibitor, and its derivatives, as well as those of other V-ATPase inhibitors, were analyzed on different HPV types in relevant cell l…
Photoaffinity cross-linking of F1ATPase from spinach chloroplasts by 3'-arylazido-beta-alanyl-8-azido ATP.
1994
UV irradiation of the ATPase (CF1) from spinach chloroplasts in the presence of 3'-arylazido-beta-alanyl-8-azido ATP (8,3'-DiN3ATP) results in a nucleotide-dependent inactivation of the enzyme and in a nucleotide-dependent formation of alpha-beta cross-links. The results demonstrate an interfacial localization of the nucleotide binding sites on CF1.
Proton Pump Inhibitors Display Antitumor Effects in Barrett's Adenocarcinoma Cells
2016
Recent evidence has reported that proton pump inhibitors (PPIs) can exert antineoplastic effects through the disruption of pH homeostasis by inhibiting vacuolar ATPase (H+-VATPase), a proton pump overexpressed in several tumor cells, but this aspect has not been deeply investigated in EAC yet. In the present study, the expression of H+-VATPase was assessed through the metaplasia-dysplasia-adenocarcinoma sequence in Barrett’s esophagus (BE) and the antineoplastic effects of PPIs and cellular mechanisms involved were evaluated in vitro. H+-VATPase expression was assessed by immunohistochemistry in paraffined-embedded samples or by immunofluorescence in cultured BE and EAC cell lines. Cells we…
Oxidative modification impairs SERCA activity in Drosophila and human cell models of Parkinson's disease
2021
DJ-1 is a causative gene for familial Parkinson's disease (PD) with different functions, standing out its role against oxidative stress (OS). Accordingly, PD model flies harboring a mutation in the DJ-1β gene (the Drosophila ortholog of human DJ-1) show high levels of OS markers like protein carbonylation, a common post-translational modification that may alter protein function. To increase our understanding of PD pathogenesis as well as to discover potential therapeutic targets for pharmacological intervention, we performed a redox proteomic assay in DJ-1β mutant flies. Among the proteins that showed increased carbonylation levels in PD model flies, we found SERCA, an endoplasmic reticulum…
Velobraucēju kustības trajektoriju analīze, izmantojot iOS Vision attēlu atpazīšanu
2019
Pārprojektējot krustojumus vai tos slēdzot remontdarbu laikā, ir svarīgi organizēt satiksmi, lai tā apmierinātu esošo dalībnieku pārvietošanās plūsmu un intensitāti. Velo braucēju trajektoriju atzīmēšana ielas attēlā ir viens no uzskatāmiem pamatojumiem, plānojot šādas izmaiņas. Darbā tiek aprakstīti iespējamie risinājumi velo braucēju atpazīšanā ar konvolūciju neironu tīkliem un izsekošanā ar datorredzi. Izvēlētie risinājumi tiek izmantoti mobilās lietotnes izstrādē uz iOS operētājsistēmas ierīcēm, kas reāllaikā spētu reģistrēt velo braucēju pārvietošanos un atzīmēt veiktās trajektorijas virs videomateriāla. Šāds rezultāts tik…
Seju un logo aptazīšanas tīmekļa sistēma
2017
Šajā dokumentā ir aprakstīta seju un logo atpazīšanas tīmekļa sistēma, kas paredzēta attēlos attēlotu seju atrašanai un no šīm sejām sistēmāievietoto seju atpazīšanai, kā arī attēlos redzamo logotipu, kas ievietoti sistēmā, atpazīšanai. Sistēmai tika aprakstīta veidota tikai aizmugures daļa, jo lietotāju saskarni implementēs pasūtītājs. Sistēma realizēta, izmantojot ārējus moduļus, Docker platformā. Tā implementēta Python valodā, izmantojot Flask ietvaru.